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Detection methods of the protein CD44 in breast cancer cell

CD44 is a cell membrane receptor that links hyaluronate to the cytoskeleton  ankyrin to mediate signal transduction. CD44 also plays a role in cell migration, differentiation, and survival signaling, which is important both to normal cells and cancer cells. Blocking of CD44 decreases the malignant activities of the tumor.

The expression of CD44 protein in human breast cancer cell line MCF-7/CD44st cells (MCF-7 cells transfected with pcDNA3.1-CD44st), MCF-7 cells, and MCF-7/neo cells (MCF-7 cells transfected with pcDNA3.1) were detected using flow cytometry analysis. Among these cell lines, only MCF-7/CD44st cells expressed a high level CD44 protein and the cells transfected stablely with pcDNA3.1-CD44st were constructed successfully.

This detection method contribute to demonstrate that HA could interact with the new CD44st and regulate the expression of MMP-2 and MMP-9, which could increase the invasion capability of MCF-7 cells through the Ras/MAPK signaling pathway.

 

Reference: Fang XJ, Jiang H, Zhao XP, Jiang WM. The role of a new CD44st in increasing the invasion capability of the human breast cancer cell line MCF-7BMC Cancer 2011, 11(1):290.