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Cell Sorting of Pneumocystis carini

Pneumocystis jirovecii provokes a severe pneumonia (PcP), which continues to be of great concern inimmunocompromised HIV-negative individuals such as patients with primary immunodeficiency, patients receiving immunosup-pressive therapies for malignancies, organ transplantations orautoimmune diseases. Using the fungal species infecting the rat, and sorting its life cycle stages by flow cytometry in order to study their transmission efficiency, their transcriptome or their ploidy level, is a step way further to understanding its life cycle.

The result showed that most of P.carinii organisms were haploid. In the cystic form, eight contents of DNA were measured thus strengthening the fact that each mature cyst contains eight haploid spores and each spore evolves into a trophic form. The ploidy data of P. carinii lifecycle stages shed new light on the complexity of its modes of proliferation.

Figure 1 Representative cell cycle analysis histograms of sorted P. carinii organisms. DNA contents of (A) all sorted P. carinii life cyclestages; (B) sorted P. carinii trophic forms; (C) haploid S. cerevisiae reference strain; (D) sorted P. carinii cystic forms; (E) diploid S. cerevisiae referencestrain.

Figure 2 Interpretation scheme of P. carinii life cycle and cell cycle.

Reference: Anna Martinez, El Moukhtar Aliouat, Annie Standaert-Vitse, et al. Ploidy of Cell-Sorted Trophic and Cystic Forms of Pneumocystis carinii. PLoS One. 2011;6(6):e20935.